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https://hdl.handle.net/20.500.13091/600
Title: | Ethylenediamine grafted carbon nanotube aerogels modified screen-printed electrode for simultaneous electrochemical immunoassay of multiple tumor markers | Authors: | Filik, Hayati Avan, Asiye Aslıhan Puntar, Nilay Altaş Özyürek, Mustafa Güngör, Zeynep Banu Kucur, Mine Dicle, Diclehan Ali Kamış, Handan |
Keywords: | Alpha-Fetoprotein Carcinoembryonic Antigen Immunosensor Electrochemistry Serum Analysis Carcinoembryonic Antigen Cancer-Detection 4 Biomarkers Silver Nanocomposites Immunosensors Polymer Lead |
Publisher: | ELSEVIER SCIENCE SA | Abstract: | A sensitive sandwich-model immunoassay designed for simultaneous electrochemical measurement of alphafetoprotein and carcinoembryonic antigen handling multiple-label strategy. The sandwich-model immunosensor was constructed by assembling ethylenediamine-MWCNT aerogels (EDA-CAGs), AuNPs and capture antibodies (Cp-Ab1) on the screen-printed carbon electrode (SPCE). The prepared EDA-CAG textures were analyzed using Fourier transform infrared (FT-IR), Scanning Electron Microscopy (SEM) and Energy Dispersive X-Ray Spectroscopy (EDX). In this protocol, AuNPs decorated EDA-CAG-carried Thionine (Thi) and AuNPs decorated EDA-CAG-carried Safranine O (SfO) (denoted as AuNP-Thi-EDA-CAG and AuNP-SfOEDA-CAG, respectively) were properly exploited as distinguishable signal labels, which were used to attach detection antibodies (Ab2) (anti-AFP2 and anti-CEA2), respectively. When two tumor antigens were present, a square wave voltammetry (SWV) scan displayed two well-separated signals, each signal indicated one target antigen. Observed results introduced that the square wave voltammetric peak current exhibited a good linear relationship to logarithm concentration in the ranges from 0.005 to 1.0 ng/mL for both analytes. The LoDs were 0.0015 and 0.0010 ng/mL (at signal/noise S/N = 3) for AFP and CEA, respectively. The designed sandwich-type immunosensor was applied to real serum sample analysis. | URI: | https://doi.org/10.1016/j.jelechem.2021.115700 https://hdl.handle.net/20.500.13091/600 |
ISSN: | 1572-6657 1873-2569 |
Appears in Collections: | Mühendislik ve Doğa Bilimleri Fakültesi Koleksiyonu Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collections WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collections |
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1-s2.0-S1572665721007268-main.pdf Until 2030-01-01 | 2.72 MB | Adobe PDF | View/Open Request a copy |
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