Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.13091/600
Title: Ethylenediamine grafted carbon nanotube aerogels modified screen-printed electrode for simultaneous electrochemical immunoassay of multiple tumor markers
Authors: Filik, Hayati
Avan, Asiye Aslıhan
Puntar, Nilay Altaş
Özyürek, Mustafa
Güngör, Zeynep Banu
Kucur, Mine
Dicle, Diclehan Ali
Kamış, Handan
Keywords: Alpha-Fetoprotein
Carcinoembryonic Antigen
Immunosensor
Electrochemistry
Serum Analysis
Carcinoembryonic Antigen
Cancer-Detection
4 Biomarkers
Silver
Nanocomposites
Immunosensors
Polymer
Lead
Issue Date: 2021
Publisher: ELSEVIER SCIENCE SA
Abstract: A sensitive sandwich-model immunoassay designed for simultaneous electrochemical measurement of alphafetoprotein and carcinoembryonic antigen handling multiple-label strategy. The sandwich-model immunosensor was constructed by assembling ethylenediamine-MWCNT aerogels (EDA-CAGs), AuNPs and capture antibodies (Cp-Ab1) on the screen-printed carbon electrode (SPCE). The prepared EDA-CAG textures were analyzed using Fourier transform infrared (FT-IR), Scanning Electron Microscopy (SEM) and Energy Dispersive X-Ray Spectroscopy (EDX). In this protocol, AuNPs decorated EDA-CAG-carried Thionine (Thi) and AuNPs decorated EDA-CAG-carried Safranine O (SfO) (denoted as AuNP-Thi-EDA-CAG and AuNP-SfOEDA-CAG, respectively) were properly exploited as distinguishable signal labels, which were used to attach detection antibodies (Ab2) (anti-AFP2 and anti-CEA2), respectively. When two tumor antigens were present, a square wave voltammetry (SWV) scan displayed two well-separated signals, each signal indicated one target antigen. Observed results introduced that the square wave voltammetric peak current exhibited a good linear relationship to logarithm concentration in the ranges from 0.005 to 1.0 ng/mL for both analytes. The LoDs were 0.0015 and 0.0010 ng/mL (at signal/noise S/N = 3) for AFP and CEA, respectively. The designed sandwich-type immunosensor was applied to real serum sample analysis.
URI: https://doi.org/10.1016/j.jelechem.2021.115700
https://hdl.handle.net/20.500.13091/600
ISSN: 1572-6657
1873-2569
Appears in Collections:Mühendislik ve Doğa Bilimleri Fakültesi Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collections
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collections

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