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https://hdl.handle.net/20.500.13091/5873
Title: | Characterization of Polysaccharide Extracts of Four Edible Mushrooms and Determination of In Vitro Antioxidant, Enzyme Inhibition and Anticancer Activities | Authors: | Deveci, Ebru Tel-Cayan, Gülsen Cayan, Fatih Altinok, Bahar Yilmaz Aktaş, Sinan |
Keywords: | Structural-Characterization Biological-Activities Ganoderma-Lucidum Alpha-Glucosidase Fruiting Bodies Purification Quantitation Antitumor |
Publisher: | Amer Chemical Soc | Abstract: | Mushroom polysaccharides are important bioactive compounds derived from mushrooms with various beneficial properties. In this study, the chemical characterization and bioactivities of polysaccharide extracts from four different edible mushrooms, Clavariadelphus truncatus Donk, Craterellus tubaeformis (Fr.) Quel., Hygrophorus pudorinus (Fr.) Fr., and Macrolepiota procera (Scop.) Singer were studied. Glucose (13.24-56.02%), galactose (14.18-64.05%), mannose (2.18-18.13%), fucose (1.21-5.78%), and arabinose (0.04-5.43%) were identified in all polysaccharide extracts by GC-MS (gas chromatography-mass spectrometry). FT-IR (Fourier transform infrared spectroscopy) confirmed the presence of characteristic carbohydrate patterns. H-1 NMR suggested that all polysaccharide extracts had alpha- and beta-d-mannopyranose, d-glucopyranose, d-galactopyranose, alpha-l-arabinofuranose, and alpha-l-fucopyranose residues. Approximate molecular weights of polysaccharide extracts were determined by HPLC (high-performance liquid chromatography). The best antioxidant activity was found in M. procera polysaccharide extract in DPPH center dot (1,1-diphenyl-2-picrylhydrazyl) scavenging (39.03% at 800 mu g/mL), CUPRAC (cupric reducing antioxidant capacity) (A(0.50): 387.50 mu g/mL), and PRAP (phosphomolybdenum reducing antioxidant power) (A(0.50): 384.08 mu g/mL) assays. C. truncatus polysaccharide extract showed the highest antioxidant activity in ABTS(center dot+) scavenging (IC50: 734.09 mu g/mL), beta-carotene-linoleic acid (IC50: 472.16 mu g/mL), and iron chelating (IC50: 180.35 mu g/mL) assays. Significant anticancer activity was found in C. truncatus polysaccharide extract on HT-29 (IC50: 46.49 mu g/mL) and HepG2 (IC50: 48.50 mu g/mL) cell lines and H. pudorinus polysaccharide extract on the HeLa cell line (IC50: 51.64 mu g/mL). Also, H. pudorinus polysaccharide extract possessed prominent AChE (acetylcholinesterase) inhibition activity (49.14% at 200 mu g/mL). | URI: | https://doi.org/10.1021/acsomega.4c00322 https://hdl.handle.net/20.500.13091/5873 |
ISSN: | 2470-1343 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collections Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collections WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collections |
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