Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.13091/969
Title: Urease immobilized electrospun PVA/chitosan nanofibers with improved stability and reusability characteristics: an application for removal of urea from artificial blood serum
Authors: Kutlu, Nur
Doğaç, Yasemin İspirli
Deveci, İlyas
Teke, Mustafa
Keywords: Chitosan
Electrospinning
Immobilization
Nanocomposites
Nanofiber
Polyvinyl Alcohol
Urease
Covalent Immobilization
Chitosan Nanocomposites
Horseradish-Peroxidase
Lipase Immobilization
Glycidyl Methacrylate
Polyvinyl-Alcohol
Pancreatic Lipase
Blend Nanofibers
Arylamine Glass
Candida-Rugosa
Issue Date: 2020
Publisher: TAYLOR & FRANCIS INC
Abstract: Electrospun polyvinyl alcohol (PVA)/Chitosan nanofibers were successfully prepared and were used as carriers for the first time in urease immobilization. Also, urease immobilized electrospun PVA/Chitosan nanofibers were applied for the removal of urea from artificial blood serum by recycled reactor. The nanofibers were optimized and synthesized by electrospinning technique according to the operational parameters. The morphology and structure of the nanofibers were characterized by scanning electron microscopy (SEM), attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) and thermogravimetric analysis (TGA). Urease was immobilized on the nanofibers by adsorption and crosslinking methods. According to immobilization results, nanofiber enhanced urease stability properties like thermal stability, pH stability, and reusability. Urease immobilized electrospun PVA/Chitosan nanofiber protected its activity by 85% after 10 uses and 45% after 20 uses. Urea removal rates of artificial blood serum were as follows: 100% at 1st cycle, 95% at 2nd, 3rd and 4th cycles; 85% at the 5th cycle; 76% at the 6th cycle, and 65% at the last three cycles.
URI: https://doi.org/10.1080/10826068.2019.1679175
https://hdl.handle.net/20.500.13091/969
ISSN: 1082-6068
1532-2297
Appears in Collections:Mühendislik ve Doğa Bilimleri Fakültesi Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collections
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collections
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collections

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