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Title: Chemical Vapor Deposition of Poly(hydroxyethyl methacrylateglycidyl methacrylate) Thin Film Coatings for Immobilization of Human Serum Albumin
Authors: Sarıipek, Fatma Bayram
Çağıl maltaş, Esra
Karaman, Mustafa
Fakülteler, Mühendislik ve Doğa Bilimleri Fakültesi, Kimya Mühendisliği Bölümü
Fakülteler, Mühendislik ve Doğa Bilimleri Fakültesi, Kimya Mühendisliği Bölümü
Keywords: iCVD
Thin film hydrogel membrane
Crosslinked PVA
Protein immobilization
Issue Date: 2019
Abstract: In this study, poly(2-hydroxyethyl methacrylate-glycidyl methacrylate) (P(HEMA-GMA) thin films were deposited on crosslinked polyvinyl alcohol (PVA) supports by initiated chemical vapor deposition (iCVD). Use of the tert-butyl peroxide as an initiator allowed very high deposition rates up to 125 nm/min at a filament temperature of 280 oC. FTIR and XPS analyses of the deposited films confirmed that the epoxide functionality of the copolymers increased with increasing glycidyl methacrylate fraction in the reactor inlet. The potential of the glycidyl methacrylate containing copolymer films to act as substrates for protein immobilization was revealed. The immobilization of Human Serum Albumin (HSA), which is the main protein to carry fatty acids and metals to target tissues, was carried out via solid phase extraction. The effects of film composition and thickness on binding capacities of the protein to the polymers were studied. The maximum protein binding for the iCVD synthesized copolymer films was found to be 223 µ . Protein binding was also clarified by FTIR, AFM, and SEM analyses. The mild immobilization conditions, easy and rapid membrane preparation, one-step protein binding at substantially higher levels and membrane reusability make iCVD deposited P(HEMAGMA) films useful for biomolecules immobilization and for several biochemical processes.
Appears in Collections:Mühendislik ve Doğa Bilimleri Fakültesi Koleksiyonu

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