Please use this identifier to cite or link to this item:
https://hdl.handle.net/20.500.13091/2501
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Telli, E. | - |
dc.contributor.author | Uçar, G. | - |
dc.contributor.author | Sönmez, G. | - |
dc.contributor.author | Telli, N. | - |
dc.contributor.author | Türkal, G. | - |
dc.contributor.author | Doğruer, Y. | - |
dc.date.accessioned | 2022-05-23T20:23:44Z | - |
dc.date.available | 2022-05-23T20:23:44Z | - |
dc.date.issued | 2021 | - |
dc.identifier.issn | 1612-9199 | - |
dc.identifier.uri | https://doi.org/10.1399/eps.2021.337 | - |
dc.identifier.uri | https://hdl.handle.net/20.500.13091/2501 | - |
dc.description.abstract | This study aimed to evaluate the viability of thermotolerant Campylobacter spp. in chicken carcasses during the slaughter process (n = 150) and at retail level (n = 72). All samples were analysed by quantitative PCR (qPCR), propidium monoazide (PMA)-treated qPCR, and direct plate counting (DPC). The quantitative comparisons were performed for assessing viability and culturability according to the values of the samples that were found to be positive in all methods. Due to the underestimation of viable but unculturable (VBNC) or dead cells, PMA-untreated samples, either at different slaughter stages or at retail level, had a higher positivity and quantity rate (p < 0.05) than the PMA-treated and DPC samples. Across the slaughter line, the above mentioned three methods revealed the highest positivity and quantity level during the evisceration stage. After the water-chilling and storage phases, positivity and quantity rates were not comparable as PMA-qPCR and DPC failed to detect the level of contamination not only in terms of cultivability but also in terms of viability as a result of compromised membrane integrity at low temperatures. In conclusion, recording of Campylobacter spp. during the chicken slaughter process by PMA-treated qPCR would provide more realistic quantitative data for poultry meat contamination. | en_US |
dc.description.sponsorship | Scientific Research Projects Coordination Unit of Selcuk University [18401024] | en_US |
dc.description.sponsorship | This study was supported by the Scientific Research Projects Coordination Unit of Selcuk University under Grant [18401024]. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Eugen Ulmer Gmbh Co | en_US |
dc.relation.ispartof | European Poultry Science | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | direct plate counting | en_US |
dc.subject | propidium monoazide | en_US |
dc.subject | retail | en_US |
dc.subject | slaughter stages | en_US |
dc.subject | viability | en_US |
dc.subject | culturability | en_US |
dc.subject | Real-Time Pcr | en_US |
dc.subject | Propidium Monoazide | en_US |
dc.subject | Chicken Carcasses | en_US |
dc.subject | Escherichia-Coli | en_US |
dc.subject | Contamination | en_US |
dc.subject | Quantification | en_US |
dc.subject | Chain | en_US |
dc.subject | Meat | en_US |
dc.subject | Pma | en_US |
dc.subject | Extraction | en_US |
dc.title | Determination of thermotolerant Campylobacter spp. by viability-qPCR in a poultry slaughterhouse and in retail | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1399/eps.2021.337 | - |
dc.identifier.scopus | 2-s2.0-85122181848 | en_US |
dc.department | Meslek Yüksekokulları, Teknik Bilimler Meslek Yüksekokulu, Gıda İşleme Bölümü | en_US |
dc.authorid | /0000-0002-4946-3749 | - |
dc.identifier.volume | 85 | en_US |
dc.identifier.wos | WOS:000707631500001 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.scopusquality | Q3 | - |
item.fulltext | No Fulltext | - |
item.openairetype | Article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
Appears in Collections: | Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collections Teknik Bilimler Meslek Yüksekokulu Koleskiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collections |
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