Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.13091/1613
Title: Comparison of microbiota and volatile organic compounds in milk from different sheep breeds
Authors: Yusuf, B.
Ezgi, T.A.
Gonca, S.
Telli, Nihat
Gürkan, U.
Keywords: 16S rRNA
headspace solid-phase microextraction gas chromatography-mass spectrometry
metagenomic analysis
sheep milk
volatile organic compound
Issue Date: 2021
Publisher: Elsevier Inc.
Abstract: In this study, we compared the microbiota and volatile organic compounds (VOC) present in the milk obtained from 3 different sheep breeds, namely Merino, Lacaune, and Assaf. Udder milk was collected from 21 animals, 7 from each breed. Bacterial microflora was determined metagenomically by extracting the DNA from the milk and analyzing the V3-V4 region of the 16S rRNA gene. Headspace solid-phase microextraction gas chromatography-mass spectrometry method was used to analyze VOC. The metagenomic analysis revealed (for Merino, Lacaune, and Assaf milk, respectively) Firmicutes (66.32, 69.36, and 57.08%), Actinobacteria (19.09, 7.67, and 19.40%), Proteobacteria (13.76, 21.06, and 22.19%), and Bacteroidetes (0.84, 1.91, and 1.33%) phyla in the milk samples. Lactobacillus was highly abundant in the milk of 3 breeds (29.64, 43.50, and 18.70%). The genera constituting more than 2% of all bacteria in all groups were Jeotgalicoccus (7.19, 5.34, and 10.77%), Enterococcus (5.18, 9.78, and 3.64%), and Corynebacterium (4.08, 3.00, and 13.44%). A total of 32 different VOC were identified by headspace solid-phase microextration analysis with 9, 30, and 24 different compounds from Merino, Lacaune, and Assaf breeds, respectively. Although ketone was the most abundant compound in Merino milk (71.84%), hydrocarbons were the most detected in Lacaune and Assaf milk (37.18% and 55.42%, respectively). A positive correlation was found between acetone, which was detected at the highest level in all groups, with Salinicoccus, Alloiococcus, Psychrobacter, and Dietzia. In addition, a negative correlation was found between the Lactobacillus genus, detected at the highest level in all groups, with methyl cyclopentane, 3-methylheptane, octane, decane, 3,3-dimethyloctane, and dodecane. Thus, differences were observed in the bacterial microflora and VOC in the sheep milk from different breeds under different feeding and breeding conditions. © 2021 American Dairy Science Association
URI: https://doi.org/10.3168/jds.2021-20911
https://hdl.handle.net/20.500.13091/1613
ISSN: 0022-0302
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collections
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collections
Teknik Bilimler Meslek Yüksekokulu Koleskiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collections

Files in This Item:
File SizeFormat 
1-s2.0-S0022030221009115-main.pdf
  Until 2030-01-01
1.1 MBAdobe PDFView/Open    Request a copy
Show full item record

CORE Recommender

SCOPUSTM   
Citations

1
checked on Mar 18, 2023

WEB OF SCIENCETM
Citations

1
checked on Jan 30, 2023

Page view(s)

78
checked on Mar 20, 2023

Google ScholarTM

Check

Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.