Browsing by Author "Yerlikaya, Fatma Humeyra"

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Citation - WoS: 1
Citation - Scopus: 1
Insomnia Patients Have a Poor Intestinal Prognosis: Accompanied by Microbiota-Derived Short Chain Fatty Acids, Diet and Zonulin
(Pergamon-Elsevier Science Ltd, 2025) Yerlikaya, Fatma Humeyra; Onmaz, Duygu Eryavuz; Selvi, Yavuz; Topkafa, Mustafa; Sivrikaya, Abdullah; Kaya, Samet; Akdag, Fadime
Background: It is becoming increasingly clear that the relationship between sleep disturbance and gut microbiota metabolites is of great importance. Aims: This study aimed to examine the changes in microbiota metabolites, brain-derived neurotrophic factors and synaptic proteins in insomnia patients, with a particular focus on the impact of diet. Methods: A total of 41 patients with insomnia and 45 healthy individuals participated in the study. The Food Frequency Questionnaire was employed to ascertain the subjects' daily macronutrient intake over the previous month. Pittsburgh Sleep Quality Index (PSQI) and Insomnia Severity Index (ISI) were used to evaluate insomnia complaints. The concentration of faecal short-chain fatty acids (SCFAs) was quantified by gas chromatography. Serum zonulin, brain derived neurotrophic factor (BDNF), postsynaptic density protein 95 (PSD-95) and synaptophysin-like protein 1 (SYPL1) protein levels were quantified using an enzyme-linked immunosorbent assay (ELISA) method. Results: The total SCFAs, acetic acid, propionic acid, butyric acid and valeric acid levels were found to be significantly lower in the insomnia patient group compared to the control group. The levels of zonulin, PSD-95 and SYPL1 were found to be significantly elevated in the insomnia patient group in comparison to the control group. A significant negative correlation was observed between PSQI and ISI values and fatty acids. Conclusions: It has been demonstrated that sleep deprivation may be associated with alterations in the metabolites produced by the gut microbiota. In Western countries where dietary fibre consumption is low, increasing SCFA levels, promoting gut integrity and homeostasis may be regarded as a promising new approach for the treatment of diseases such as insomnia.
Investigation of Intestinal Microbiota and Short-Chain Fatty Acids in Colorectal Cancer and Detection of Biomarkers
(MDPI, 2025) Saylam, Esra; Ozden, Ozben; Yerlikaya, Fatma Humeyra; Sivrikaya, Abdullah; Yormaz, Serdar; Arslan, Ugur; Macin, Salih
Colorectal cancer (CRC) is one of the most common cancers worldwide and a significant global health issue. The human gut microbiota, a complex ecosystem hosting numerous microorganisms such as bacteria, viruses, fungi, and protozoa, plays a crucial role. Increasing evidence indicates that gut microbiota is involved in CRC pathogenesis. In this study, the gut microbiota profiles, short-chain fatty acids, zonulin, and lipopolysaccharide-binding protein levels of newly diagnosed CRC patients were analyzed along with healthy controls to elucidate the relationship between CRC and the gut microbiota. The study included 16 newly diagnosed CRC patients and 16 healthy individuals. For microbiota analysis, DNA isolation from stool samples was performed using the Quick-DNA (TM) Fecal/Soil Microbe Miniprep Kit followed by sequencing using the MinION device. Data processing was conducted using Guppy software (version 6.5.7) and the Python (3.12) programming language. ELISA kits from Elabscience were utilized for analyzing LBP and zonulin serum levels. Fecal short-chain fatty acids were analyzed using GC-MS/MS equipped with a flame ionization detector and DB-FFAP column. Microbial alpha diversity, assessed using Shannon and Simpson indices, was found to be lower in CRC patients compared to healthy controls (p = 0.045, 0.017). Significant differences in microbial beta diversity were observed between the two groups (p = 0.004). At the phylum level, Bacteroidota was found to be decreased in CRC patients (p = 0.027). Potential biomarker candidates identified included Enterococcus faecium, Ruminococcus bicirculans, Enterococcus gilvus, Enterococcus casseliflavus, Segatella oris, and Akkermansia muciniphila. Serum zonulin levels were higher in CRC patients (CRC = 70.1 +/- 26.14, Control = 53.93 +/- 17.33, p = 0.048). There is a significant relationship between gut microbiota and CRC. A multifactorial evaluation of this relationship could shed light on potential biomarker identification and the development of new treatment options for CRC.