Browsing by Author "Muhammad, Akhtar"

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    Isolation, Structural Characterization, and Biological Activities of Galactomannans From Rhizopogon Luteolus and Ganoderma Adspersum Mushrooms
    (ELSEVIER, 2020) Çayan, Gülsen Tel; Muhammad, Akhtar; Deveci, Ebru; Duru, Mehmet Emin; Öztürk, Mehmet
    Polysaccharides are essential compounds that contribute to the biological activities of mushrooms. Two new galactomannans (Galactomannan I and II) were isolated from R. luteolus and G. adspersum. Their structures were characterized using FT-IR, 1D, and 2D-NMR techniques. Both isolated galactomannans I and II mainly include D-mannose and D-galactose in the molar percentages of 0.81:1.0 and 1:1.4, respectively. The GPC calculation demonstrated that the molecular weights are about 5240 and 5090 Da, respectively. Their structures comprise of beta-(1,4)-mannose (Man) backbone units with alpha-(1,6)-galactose (Gal) single unit as a side group. The anticholinesterase activity of galactomannans was tested against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), spectrophotometrically. Antioxidant activity was also measured by six assays (ABTS(center dot+), DPPH center dot, O-2(center dot-), beta-carotene-linoleic acid, metal chelating, and CUPRAC assays). Galactomannan II indicated close inhibitory activity to galantamine against AChE (61.04 +/- 0.45%) and BChE (59.70 +/- 1.15%) at 50 mu g/mL concentration. Nevertheless, both galactomannans showed low antioxidant activity in all tests. This study reveals that mainly, Galactomannan II could be used as a new natural promising anticholinesterase agent. (C) 2020 Elsevier B.V. All rights reserved.