Browsing by Author "Molo, Zeynep"

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Citation - WoS: 12
Citation - Scopus: 14
Chemometrics Evaluation of Phytochemicals and Antioxidant Activities of the Extracts of Chaerophyllum Bulbosum Roots and Aerial Parts
(TAYLOR & FRANCIS INC, 2022) Çayan, Gülsen Tel; Deveci, Ebru; Çayan, Fatih; Molo, Zeynep; Duru, Mehmet Emin; Yeşil, Yeter
The objective of this study was provide the first report to assess the high-performance liquid chromatography-diode array detection (HPLC-DAD) identification of phytochemicals, the spectrophotometric determination of total phenolics and flavonoids, and antioxidant activities of the root and aerial extracts of Chaerophyllum bulbosum. HPLC-DAD identified the main phytochemical to be rosmarinic acid in the roots in the acetone (CBRA) (13.98 +/- 0.12 mg/g) and the aerial water (CBAW) extract; myricetin (8.30 +/- 0.22 mg/g) in the root methanol extract (CBRM); trans-aconitic acid (6.46 +/- 0.49 mg/g) in the root water extract (CBRW) and fumaric acid in the aerial methanol (CBAM) (17.43 +/- 0.87 mg/g) and water CBAW (18.96 +/- 0.81 mg/g) extracts. The concentrations of total phenolics and flavonoids were 2.00 +/- 0.14-17.79 +/- 0.99 mu g PEs/mg extract and 2.90 +/- 0.00-27.64 +/- 0.37 mu g QEs/mg extract, respectively. The highest antioxidant activity was found in the root acetone extract (CBRA) using the beta-carotene-linoleic (IC50: 172.31 +/- 0.16 mu g/mL) and CUPRAC (A(0.50): 126.00 +/- 0.24 mu g/mL) assays and in the root water extract (CBRW) for DPPH center dot (IC50: 59.51 +/- 0.21 mu g/mL) and metal chelating (IC50: 65.91 +/- 0.36 mu g/mL) assays in the aerial water extract (CBAW) using the ABTS(center dot+) (IC50: 59.51 +/- 0.21 mu g/mL) assay. The relationship between the phytochemicals and antioxidant activities of the extracts was chemometrically determined by principal component analysis (PCA) and hierarchical cluster analysis (HCA).
Citation - WoS: 8
Citation - Scopus: 11
Insight Into Isolation and Characterization of Compounds of Chaerophyllum Bulbosum Aerial Part With Antioxidant, Anticholinesterase, Anti-Urease, Anti-Tyrosinase, and Anti-Diabetic Activities
(ELSEVIER, 2021) Molo, Zeynep; Çayan, Gülsen Tel; Deveci, Ebru; Öztürk, Mehmet; Duru, Mehmet Emin
Chaerophyllum species are used as a food additive with their unique aroma and in traditional treatment with beneficial health effects. In this study, various extracts of Chaerophyllum bulbosum L. aerial parts were investigated in terms of the isolation of compounds and ability to inhibit enzyme (AChE, BChE urease, tyrosinase, alpha-amylase, and alpha-glucosidase) activities. The hexane extract showed higher inhibitory activity against all enzymes except urease. Nine compounds, n-octacosane (1), n-heptadecanyl eicosanoate (2), stigmasterol (3), beta-sitosterol-3-O-beta-D-glucopyranoside (4), quercetin-3-O-beta-D-glucopyranoside (5), apigenin-7-O-beta-D-glucopyr-anoside (6), luteolin-7-O-beta-D-glucopyranoside (7), sucrose (8), and luteolin-7-O-alpha-D-glucopyranoside-4 ''-O-beta-D-glucopyranoside (9) were purified from C. bulbosum and their chemical structures were defined by confirming their IR, NMR, and MS data with the literature. Antioxidant and enzyme inhibitory activities of all purified compounds were searched. Compounds 5 and 7 were found to have higher DPPH. and ABTS.+ scavenging activities than standards BHA and alpha-tocopherol. 1 (IC50: 1.63 +/- 0.05 mu g/mL), 2 (IC50: 2.02 +/- 0.13 mu g/mL) and 3 (IC50: 5.36 +/- 0.22 mu g/mL) had higher urease inhibition activity than thiourea (IC50: 7.87 +/- 0.18 mu g/mL) used as a standard. In addition, it was determined that compound 7 (IC50: 352.63 +/- 0.23 mu g/mL) exhibited higher alpha-glucosidase enzyme inhibitory activity as compared to acarbose (IC50: 378.66 +/- 0.14 mu g/mL) while it showed a competitive activity against alpha-amylase. The results reveal that C. bulbosum can be used as a natural source for developing plant-based formulations in fields such as food, pharmaceutical, and cosmetics.
Isolation and Characterization of Chemical Constituents From Chaerophyllum Bulbosum Roots and Their Enzyme Inhibitory and Antioxidant Effects
(Walter De Gruyter Gmbh, 2022) Tel-Çayan, Gülşen; Deveci, Ebru; Molo, Zeynep; Duru, Mehmet Emin; Öztürk, Mehmet
Isolation and bioactive effects of the roots of Chaerophyllum bulbosum L. were firstly investigated herein. Enzyme (acetylcholinesterase, butyrylcholinesterase, urease, alpha-amylase, alpha-glucosidase, and tyrosinase) inhibitory effects of C. bulbosum root extracts were tested. Three known compounds, n-heptadecanyl eicosanoate (1), stigmasterol (2), and beta-sitosterol-3-O-beta-d-glucopyranoside (3) were isolated from C. bulbosum. Antioxidant and enzyme inhibitory effects of isolated compounds were investigated. The hexane extract (IC50: 349.58 +/- 0.06 mu g/mL) displayed a higher alpha-glucosidase inhibitory effect than the standard (IC50: 378.66 +/- 0.14 mu g/mL). The best inhibitory effect was found in compound 2 on AChE (46.40 +/- 0.31%), BChE (56.41 +/- 0.54%), and urease (92.47 +/- 0.11%); compound 1 on alpha-amylase (22.27 +/- 0.61%); and compound 3 on alpha-glucosidase (12.43 +/- 0.25%) and tyrosinase (19.00 +/- 0.16%). All isolated compounds showed moderate antioxidant effects in all assays. This study contributes to the therapeutic uses of Chaerophyllum roots and emphasizes the value of C. bulbosum species for the development of novel therapeutic agents.