Browsing by Author "Keskinkaya, Hatice Banu"

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Citation - Scopus: 6
Chemical Composition, in Vitro Antimicrobial and Antioxidant Activities of Marine Macroalgae Codium Fragile (suringar) Hariot
(2022) Keskinkaya, Hatice Banu; Güneş, Erdoğan; Okudan, Emine Şükran; Akköz, Cengiz; Gümüş, Numan Emre; Karakurt, Serdar; Deveci, Ebru
Marine algae, which are the primary producers living in aquatic areas, are the subject of many studies due to their importance as they are eukaryotic and eutrophic organisms that play a crucial role in the pharmaceutical, cosmetic, food, fuel, and textile industries. Macroalgae are known in producing several macronutrients, micronutrients, and other important biologically active compounds (e.g. polyphenols, enzymes, and antibiotics) with potential pharmacological uses. In this research, we aimed to investigate the chemical composition, antimicrobial and antioxidant activities (with three assays), total phenolic (TPC) and flavonoid (TFC) contents of the methanol, ethanol, acetone, and water extracts of Codium fragile (Suringar) Hariot. The LC-ESI-MS/MS assessment allowed the identification of seven compounds containing gallic acid, 4-hydroxybenzaldehyde, 4-hidroxybenzoic acid, p-coumaric acid, salicylic acid, biochanin A, and diosgenin. TPC and TFC of the extracts were calculated as in the range of 10.34±0.13-64.67±0.02 µg GAEs/mg extract and 12.73±2.68-36.78±1.08 µg QEs/mg extract, respectively. All extracts of C. fragile showed antimicrobial activity against all test pathogens at different levels. The methanol, ethanol, and acetone extracts showed different levels of activity against gram-negative and gram-positive bacteria (MIC: 3.125-1.562 mg/mL). The water extract showed the highest activity in ABTS•+ (70.43±14.85%) and DPPH• (72.61±11.44%) assays while the acetone extract exhibited the best activity in CUPRAC (absorbance: 0.60±0.15) assay. The results we obtained approved that C. fragile could be valued as a natural source of bioactive agents for food preservatives and in other industrial and pharmaceutical fields.
Citation - WoS: 6
Citation - Scopus: 6
Hplc-Uv Analysis of Phenolic Compounds and Biological Activities of Padina Pavonica and Zanardinia Typus Marine Macroalgae Species
(Scientific And Technological Research Council Turkey, 2023) Keskinkaya, Hatice Banu; Deveci, Ebru; Yılmaz Altınok, Bahar; Gümüş, Numan Emre; Okudan Aslan, Emine Şükran; Akköz, Cengiz; Karakurt, Serdar
The marine macroalgae species are recognized as the food of the future with excellent bioactive properties. This study aimed to investigate phenolic compounds; cytotoxic, antibacterial, and antioxidant activities; total phenolic (TPC) and flavonoid (TFC) contents of the methanol, ethanol, and water extracts of Padina pavonica (PP) and Zanardinia typus (ZT). To the best of our knowledge, this is the first report in which ZT was evaluated in terms of phenolic content, antioxidant, antibacterial, and cytotoxic activities. The HPLC analysis allowed the identification of five phenolic compounds containing rutin (0.31 +/- 0.04 - 3.00 +/- 0.21 ppm) in all extracts and trans- p-coumaric acid (0.15 +/- 0.02 - 3.95 +/- 0.02 ppm) in MPP, EPP, MZT, EZT, WZT as the major compounds. TPC and TFC of the extracts were calculated as 11.78 +/- 0.75 - 76.78 +/- 0.54 mu g GAEs/mg extract and 6.78 +/- 0.17 - 29.50 +/- 2.23 mu g QEs/mg extract, respectively. The highest cytotoxicity was observed in EZT (CC50: 132.3 +/- 22.4 mu g/mL) against MCF-7 and MZT (CC50: 91.4 +/- 20.9 mu g/mL) against MIA PaCa-2. Among the studied extracts, EPP showed the best antibacterial activity against all test pathogens. Also, EPP indicated superior antibacterial activity against Plesiomonas shigelloides (MIC: 1.25 mg/mL) and Staphylococcus aureus (MIC: 1.25 mg/mL). EZT displayed the highest antioxidant activity in DPPH center dot (IC50: 49.03 +/- 0.28 mu g/mL), CUPRAC (A0.50: 15.20 +/- 0.14 mu g/mL), and ABTS center dot+ (IC50: 18.86 +/- 0.74 mu g/mL) assays. The results approved that P. pavonica and Z. typus marine macroalgae species could be valued as natural sources of bioactive agents for food and pharmacology applications.