Browsing by Author "Duru, Mehmet Emin"

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Citation - Scopus: 3
Anti-Colorectal Cancer Effects of Medicinal Plants: Euphorbia Helioscopia, Ferula Elaeochytris, and Sideritis Albiflora
(2021) Deveci, Ebru; Çayan, Gülsen Tel; Karakurt, Serdar; Duru, Mehmet Emin
Phytochemicals, extracts, and mixtures obtained from plants have been offered as an option for cancer treatment and prevention for modern drug discovery in recent years. For this purpose, in this study, anti-colorectal cancer effects of the hexane, acetone, methanol, and water extracts obtained by sequential extraction from Euphorbia helioscopia L., Ferula elaeochytris Korovin, and Sideritis albiflora Hub.-Mor. on DLD-1 cell line were investigated in vitro by using Alamar blue assay. Dosedependent inhibition was detected in the viability of DLD-1 cell line. In all three plants species, E. helioscopia (IC50: 140.83±0.31 µg/mL), F. elaeochytris (IC50: 67.93±0.12 µg/mL), and S. albiflora (IC50: 85.12±0.10 µg/mL) methanol extracts showed higher anti-colorectal effects on DLD-1 cell line compared to other extracts tested for the same species. In addition, the IC50 value of doxorubicin used as a standard was found as 6.10±0.55 µg/mL. With the results obtained, as the first report highlighting in vitro anti-colorectal cancer effects of the studied plant species on DLD-1 cell line, promising marks were obtained from the analysis of the extracts as anti-cancer sources for plant-derived drug applications.
Citation - Scopus: 11
Antioxidant, Cytotoxic, and Enzyme Inhibitory Activities of Agropyron Repens and Crataegus Monogyna Species
(2020) Deveci, Ebru; Çayan, Gülşen Tel; Karakurt, Serdar; Duru, Mehmet Emin
Objective: The aim of this study was to investigate antioxidant, enzyme inhibitory and cytotoxic activities of Agropyron repens and Crataegus monogyna methanol extracts with total phenolic and flavonoid contents. Materials and Methods: Total phenolic and flavonoid contents of A. repens and C. monogyna methanol extracts were measured according to Folin Ciocalteu and aluminum nitrate methods, respectively. Antioxidant and enzyme inhibitory activities of the methanol extracts were tested spectrophotometrically. Also, cytotoxic activities of the methanol extracts against DLD-1 and CCD-18Co were investigated by using Alamar Blue assay. Results: C. monogyna methanol extract with the highest total phenolic and flavonoid contents (68.13±0.34 µg GAEs/mg extract and 36.91±0.17 µg QEs/mg extract, respectively) had the best antioxidant activity in ?-carotene-linoleic acid ($IC_50$: 32.72±0.15 µg/mL), CUPRAC ($A_0.50$: 282.69±0.25 µg/mL), DPPH• ($IC_50$: 71.69±0.85 µg/mL), and ABTS•+ ($IC_50$: 40.43±0.55 µg/ mL) assays. A. repens methanol extract showed the highest effect against AChE (18.73±0.47 %), BChE (37.59±1.07 %), urease (89.18±0.84%), ?-glucosidase (6.71±0.23 %), whereas C. monogyna methanol extract showed the highest effect against tyrosinase (30.52±1.00%) and ?-amylase (37.24±0.06 %). Also, A. repens ($IC_50$: 57.38 µg/mL) and C. monogyna ($IC_50$: 54.04 µg/ mL) methanol extracts showed close cytotoxic activity on DLD-1. Conclusion: Antioxidant, cytotoxic, and enzyme inhibitory activities of A. repens and C. monogyna methanol extracts were investigated with total phenolic and flavonoid contents in this study. The results obtained with this study strengthen the potential of the studied plants as a new source for the discovery of antioxidant, cytotoxic, and enzyme inhibitor agents.
Assessment of Cytotoxic Effect of Porodaedalea Pini Mushroom on Prostate Cancer
(2022) Deveci, Ebru; Duru, Mehmet Emin; Çayan, Gülsen Tel
Phellinus species are in the classification of valuable mushrooms among medicinal mushrooms and have been the source of traditional medicine for centuries. In this study, the hexane (PPH), methanol (PPM) and water (PPW) extracts, fractions and isolated compounds from Porodaedalea pini (Çamkavı mantarı) were screened for their cytotoxic effects on PC3 (prostate cancer) and 3T3 (murine fibroblast) cell lines by MTT assay. PPH was found to have moderate cytotoxicity on PC3 cell with the IC50 values of 33.84±0.01 µg/mL. The fatty acid profile of PPH was identified by GC-FID (gas chromatography-flame ionization dedector) and GC-MS (gas chromatography-mass spectrometry) and the main fatty acids were recorded as palmitic (41.40±0.03%), linoleic (21.53±0.01%), oleic (12.06±0.01%), and stearic (8.20±0.01%) acids. PPM and PPW were fractioned by using liquid-liquid extractions. Among all fractions, the 2-butanol fraction of the methanol extract (PPM-B) indicated the best cytotoxicity on PC3 and 3T3 cell lines. Also, among the isolated compounds (dioctyl phthalate (1), ergosterol peroxide (2) and pinoresinol (3)) from PPM, ergosterol peroxide (2) was found as moderate cytotoxic on PC3 with the IC50 value of 95.47±1.01 µg/mL. This study, in which the effect of P. pini on PC3 cell was examined for the first time, proved its medicinal importance by revealing the cytotoxic properties of the species.
Citation - WoS: 13
Citation - Scopus: 15
Chemometric Approaches for the Characterization of the Fatty Acid Composition of Seventeen Mushroom Species
(TAYLOR & FRANCIS INC, 2020) Çayan, Fatih; Deveci, Ebru; Çayan, Gülsen Tel; Duru, Mehmet Emin
Mushrooms have been used since ancient times because of their nutritional, medicinal and economic potential. In this study, fatty acid compositions of seventeen mushroom species, naturally growing in Turkey, were determined by using gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Totally, twenty-four fatty acids were identified in mushroom species. Generally, oleic (6.76-59.25%), linoleic (6.45-61.63%), palmitic (3.57-28.09%) and stearic (0.90-19.77%) acids were identified as the main fatty acids in all studied mushroom species. Principal component analyses (PCA) and hierarchical clustering analyses (HCA) were applied to analyze the chemometry of twenty-four fatty acids of the seventeen mushroom species by using Minitab 16.0 software. The main fatty acid compounds found in the mushrooms and their concentrations have been effective in distinguishing mushroom species from each other. As a result of PCA and HCA analysis, it was determined that Daedalea quercina (DQ) was distinctly separated from other mushroom species in terms of fatty acid composition. This study reveals that the chemometric analysis by PCA and HCA techniques could be used for the classification of the mushroom species according to their fatty acid compositions.
Citation - WoS: 12
Citation - Scopus: 14
Chemometrics Evaluation of Phytochemicals and Antioxidant Activities of the Extracts of Chaerophyllum Bulbosum Roots and Aerial Parts
(TAYLOR & FRANCIS INC, 2022) Çayan, Gülsen Tel; Deveci, Ebru; Çayan, Fatih; Molo, Zeynep; Duru, Mehmet Emin; Yeşil, Yeter
The objective of this study was provide the first report to assess the high-performance liquid chromatography-diode array detection (HPLC-DAD) identification of phytochemicals, the spectrophotometric determination of total phenolics and flavonoids, and antioxidant activities of the root and aerial extracts of Chaerophyllum bulbosum. HPLC-DAD identified the main phytochemical to be rosmarinic acid in the roots in the acetone (CBRA) (13.98 +/- 0.12 mg/g) and the aerial water (CBAW) extract; myricetin (8.30 +/- 0.22 mg/g) in the root methanol extract (CBRM); trans-aconitic acid (6.46 +/- 0.49 mg/g) in the root water extract (CBRW) and fumaric acid in the aerial methanol (CBAM) (17.43 +/- 0.87 mg/g) and water CBAW (18.96 +/- 0.81 mg/g) extracts. The concentrations of total phenolics and flavonoids were 2.00 +/- 0.14-17.79 +/- 0.99 mu g PEs/mg extract and 2.90 +/- 0.00-27.64 +/- 0.37 mu g QEs/mg extract, respectively. The highest antioxidant activity was found in the root acetone extract (CBRA) using the beta-carotene-linoleic (IC50: 172.31 +/- 0.16 mu g/mL) and CUPRAC (A(0.50): 126.00 +/- 0.24 mu g/mL) assays and in the root water extract (CBRW) for DPPH center dot (IC50: 59.51 +/- 0.21 mu g/mL) and metal chelating (IC50: 65.91 +/- 0.36 mu g/mL) assays in the aerial water extract (CBAW) using the ABTS(center dot+) (IC50: 59.51 +/- 0.21 mu g/mL) assay. The relationship between the phytochemicals and antioxidant activities of the extracts was chemometrically determined by principal component analysis (PCA) and hierarchical cluster analysis (HCA).
Citation - WoS: 14
Citation - Scopus: 16
A Comprehensive Study on Phenolic Compounds and Bioactive Properties of Five Mushroom Species Via Chemometric Approach
(WILEY, 2021) Çayan, Fatih; Çayan, Gülsen Tel; Deveci, Ebru; Duru, Mehmet Emin
This study was planned to investigate the phenolic compounds, cholinesterase inhibitory, and antioxidant activities of Chondrostereum purpureum (CP), Hymenochaete rubiginosa (HR), Macrolepiota procera (MP), Phaeolus schweinitzii (PS), and Phellinus igniarius (PI) mushroom species. High-performance liquid chromatography (HPLC-DAD) was used for the characterization of the phenolic compounds. The main compound was identified as fumaric acid in C. purpureum (CP), M. procera (MP), and P. igniarius (PI); protocatechuic acid in H. rubiginosa (HR); 6,7-dihydroxy coumarin in P. schweinitzii (PS). P. schweinitzii methanol (PSM) extract had close or higher activity than standards in ABTS(center dot+) (IC50: 2.96 +/- 0.15 mu g/ml), DPPH center dot (80.56 +/- 0.53%), CUPRAC (A(0.50): 5.26 +/- 0.19 mu g/ml), beta-carotene-linoleic acid (IC50: 1.16 +/- 0.14 mu g/ml) assays. In addition, P. schweinitzii methanol (PSM) extract showed potential inhibitory activity against AChE (56.38 +/- 0.09%). The relationship between the phenolic compounds, antioxidant, and cholinesterase inhibitory activity results and the mushroom species were examined using principal component analysis (PCA) and hierarchical cluster analysis (HCA). Practical applications This study revealed that studied mushroom species have significant antioxidant and cholinesterase inhibitory activities and are found to be rich in phenolic compounds. The mushroom species were clustered with their antioxidant and cholinesterase inhibitory activities and phenolic compounds data according to the PCA and HCA analysis. Overall, the results described in this study increase the contribution and emphasis on the potential use of these mushrooms as a source of versatile biological agents.
Citation - Scopus: 6
Cytotoxic Activities of Methanol Extract and Compounds of Porodaedalea Pini Against Colorectal Cancer
(2021) Deveci, Ebru; Çayan, Gülsen Tel; Karakurt, Serdar; Duru, Mehmet Emin
Porodaedalea pini is a medicinally important mushroom with antioxidant, cytotoxic, immunostimulating, antitumor, antiviral and immunomodulating activities. Therefore, in this study, P. pini methanol extract and isolated compounds from the methanol extract were tested for cytotoxic activities against DLD-1 (colorectal cancer) and CCD-18Co (human colon fibroblast cell line) by using Alamar Blue assay. Cytotoxic activity on DLD-1 was decreased in the order of P. pini methanol extract> 4-(3,4- dihydroxyphenyl)but-3-en-2-one (3)> pinoresinol (2)> ergosta-7,24(28)-dien3?-ol (1). P. pini methanol extract was determined to have the best cytotoxic activity with the lowest IC50 value on DLD-1 (IC50: 25.33±0.29 µg/mL) and the highest IC50 value on CCD-18Co (434.30±1.45 µg/mL). Within the scope of the findings, it is thought that P. pini mushroom can be used as a new and natural agent in the treatment of colorectal cancer.
Citation - WoS: 10
Citation - Scopus: 16
A Detailed Study on Multifaceted Bioactivities of the Extracts and Isolated Compounds From Truffle Reddellomyces Parvulosporus
(WILEY, 2022) Çayan, Fatih; Çayan, Gülsen Tel; Deveci, Ebru; Duru, Mehmet Emin; Türk, Mustafa
Mushrooms and truffles are attracting attention as a new generation of biotherapeutics. In the current study, isolation, phenolic and organic acid composition, and antioxidant, cytotoxic, anticholinesterase activities of truffle Reddellomyces parvulosporus were examined. Four known compounds (brassicasterol (1), ergosterol peroxide (2), fumaric acid (3) and mannitol (4)) were isolated with the combination of chromatographic techniques. Fumaric acid (54.74 +/- 0.85 mu g g(-1)) was found as the major compound by HPLC-DAD. All isolated compounds were bioassayed for antioxidant, cytotoxic, anticholinesterase, anti-tyrosinase, anti-urease, anti-alpha-glucosidase and anti-alpha-amylase activities. Compound 1 indicated notable cytotoxic activity on MCF-7 (IC50: 38.08 +/- 0.75 mu g mL(-1)) and compound 3 on H1299 (IC50: 62.37 +/- 0.75 mu g mL(-1)). Also, compounds 1 (84.55 +/- 1.14%) and 2 (84.90 +/- 0.10%) showed higher anti-urease activity than thiourea (78.57 +/- 0.22%), while compound 2 (66.31 +/- 0.08%) displayed near-standard anti-BChE activity. Also, being the first to emphasise the potential of R. parvulosporus as a natural food additive, this study evidenced its medicinal importance by revealing bioactive compounds and properties.
Citation - WoS: 21
Citation - Scopus: 22
Hplc-Dad Characterization of Phenolic Profile and in Vitro Antioxidant, Anticholinesterase, and Antidiabetic Activities of Five Mushroom Species From Turkey
(Springer Heidelberg, 2021) Çayan, Fatih; Tel-Çayan, Gülşen; Deveci, Ebru; Duru, Mehmet Emin
In this study, Daedalea quercina (L.) Pers., Hydnum repandum L., Inonotus radiatus (Sowerby) P. Karst., Omphalotus olearius (DC.) Singer, and Schizophyllum commune Fr. hexane and methanol extracts were subjected to the spectrophotometric assays for antioxidant and enzyme inhibitory activities, which are linked with human diseases that are very prevalent in recent years. Additionally, phenolic compounds of the mushrooms were quantified by HPLC-DAD. The best antioxidant activity was found in H. repandum methanol extract (IC50: 12.04 +/- 0.24 mu g/mL) in the beta -carotene-linoleic assay; I. radiatus methanol extract in DPPH center dot (81.22 +/- 0.50%), ABTS(center dot+) (IC50: 73.47 +/- 0.18 mu g/mL), and CUPRAC (A(0.50): 88.21 +/- 0.02 mu g/mL) assays; S. commune hexane extract (53.36 +/- 0.89%) in the metal chelating assay. O. olearius hexane extract was found as the best inhibitor against AChE (71.58 +/- 0.28%) and BChE (67.30 +/- 0.15%). When I. radiatus methanol (95.88 +/- 0.74%) and H. repandum hexane (95.75 +/- 0.16%) extracts showed close alpha -amylase inhibitory activity to acarbose (96.68 +/- 0.08%), D. quercina methanol extract (70.79 +/- 0.34%) had higher alpha -glucosidase inhibitory activity than acarbose (67.01 +/- 2.28%). Among 16 phenolic compounds analyzed, gallic acid (0.02 +/- 0.01-0.23 +/- 0.01 mu g/g) was detected in all studied mushrooms. This study provides that investigated mushrooms can be used for further research, which can lead to the development of new natural remedies to alleviate complications related to oxidative stress, diabetes, and neurological diseases.
Citation - WoS: 83
Citation - Scopus: 98
Identification and Quantification of Phenolic Acid Compounds of Twenty-Six Mushrooms by Hplc-Dad
(SPRINGER, 2020) Çayan, Fatih; Deveci, Ebru; Çayan, Gülsen Tel; Duru, Mehmet Emin
Phenolic acids are found in different foods in the human diet, for example mushrooms. Determination of phenolic acids is important because of their relationship to their role in disease prevention due to their bioactive properties. In this study, the phenolic acid profile of 26 mushroom species was analyzed by using high-performance liquid chromatography method coupled with photodiode array detector (HPLC-DAD) and 16 phenolic acid compounds were identified. The chromatographic separation was performed using Intertsil ODS-3 reverse phase C-18 column (5 mu m, 250 mm x 4.6 mm i.d), gradient solvent system with 1.5 mL/min flow rate and detected at 280 nm. The coefficient of determination (R-2) was in the range of 0.9965-0.9999. Limit of detection and quantification ranged from 0.001-0.970 to 0.001-2.940 mu g/L, respectively. The phenolic compounds were characterized according to their retention times and UV data were compared with commercial standards. S. granulatus (71.79 mu g/g) and L. nuda (68.38 mu g/g) revealed the highest concentration of total phenolic compounds among the studied mushrooms. Gallic acid was found as the major phenolic compound in R. aurora (2.96 +/- 0.56 mu g/g) while 6,7-dihydroxy coumarin was identified as major phenolic compounds in A. tabescens (2.07 +/- 0.25 mu g/g) and L. leucothites (9.02 +/- 0.87 mu g/g). Fumaric acid was found as the most abundant compounds in 16 out of 26 mushrooms. Catechin hydrate was identified as major phenolic compounds in the rest of mushrooms. This method provided a beneficial standardization procedure of phenolic acid compounds in mushroom samples.
Citation - Scopus: 9
In Vitro Antidiabetic Activity of Seven Medicinal Plants Naturally Growing in Turkey
(2020) Deveci, Ebru; Çayan, Gülsen Tel; Duru, Mehmet Emin
Objective: Diabetes mellitus is a worldwide metabolic/endocrine disease that causes major medical problems. One of the most important strategies used in the therapy of the diabetes mellitus is the use of inhibition of ?-glucosidase and ?-amylase enzymes. Therefore, this study aimed to investigate antidiabetic activities of the hexane and methanol extracts of the medicinal plants from Turkey. Materials and Methods: The hexane and methanol extracts of Euphorbia helioscopia, Ferula elaeochytris, Sideritis albiflora, Sideritis stricta, Sideritis pisidica, Sideritis leptoclada, Salvia chionantha plants were prepared at room temperature. Antidiabetic activities of the extracts on ?-glucosidase and ?-amylase enzymes were determined. Results: S. pisidica hexane extract exhibited higher ?-amylase inhibitory activity than acarbose (96.60±0.08 %) used as a standard with an inhibition value of 97.99±0.79 % at 1000 µg/mL concentration. In terms of ?-glucosidase inhibitory activities, the extracts were ranked in the following order: F. elaeochytris hexane extract > S. leptoclada hexane extract > S. stricta hexane extract > E. helioscopia hexane extract. Conclusion: In this study, antidiabetic activities of the extracts on ?-glucosidase and ?-amylase enzymes of the studied medicinal plants were screened for the first time. It has been suggested that S. pisidica hexane extract can be used as antidiabetic agent.
Citation - WoS: 46
Citation - Scopus: 56
Inhibitory Activities of Medicinal Mushrooms on Alpha-Amylase and Alpha-Glucosidase Related To Type 2 Diabetes
(ELSEVIER, 2021) Deveci, Ebru; Çayan, Fatih; Çayan, Gülsen Tel; Duru, Mehmet Emin
Mushrooms have been used as a primary source of medicines since ancient times due to the presence of bioactive compounds. Enzyme inhibition is an important field of pharmaceutical research that provides insight into the discovery of a large variety of drugs that are useful in treating many diseases. The inhibitory activities of the hexane and methanol extracts of twenty-four medicinal mushrooms on alpha-amylase and alpha-glucosidase enzymes related to type 2 diabetes were evaluated in this study. Among all studied mushroom extracts, C. rutilus hexane extract (IC50: 0.05 +/- 0.01 mg/mL) demonstrated the highest inhibitory activity on alpha-amylase while P. ostreatus hexane (IC50: 0.10 +/- 0.01 mg/mL) showed the highest inhibitory activity on alpha-glucosidase. The hexane extracts of C. rutilus (98.81 +/- 0.01 %), G. adspersum (96.96 +/- 0.47 %), G. sepiarium (96.89 +/- 1.01 %), and S. granulatus (97.74 +/- 0.27 %) displayed higher inhibitory activity on alpha-amylase enzyme than acarbose at 1.00 mg/mL concentration. Also, the hexane extracts of P. ostreatus (IC50: 0.10 +/- 0.01 mg/mL), M. procera (IC50: 0.11 +/- 0.01 mg/mL), P. schweinitzii (IC50: 0.14 +/- 0.01 mg/mL), L. gentianeus (IC50: 0.22 +/- 0.02 mg/mL), P. pini (IC50: 0.22 +/- 0.03 mg/mL) and the methanol extracts of T. pubescens (IC50: 0.12 +/- 0.02 mg/mL) and G. adspersum (IC50: 0.20 +/- 0.04 mg/mL) showed higher inhibitory activity on alpha-glucosidase enzyme than acarbose (IC50: 0.37 +/- 0.01 mg/mL). This study with the reported results supported the use of mushrooms in the pharmaceutical industries as natural antidiabetic agents through key enzyme inhibition. (C) 2020 SAAB. Published by Elsevier B.V. All rights reserved.
Citation - WoS: 8
Citation - Scopus: 11
Insight Into Isolation and Characterization of Compounds of Chaerophyllum Bulbosum Aerial Part With Antioxidant, Anticholinesterase, Anti-Urease, Anti-Tyrosinase, and Anti-Diabetic Activities
(ELSEVIER, 2021) Molo, Zeynep; Çayan, Gülsen Tel; Deveci, Ebru; Öztürk, Mehmet; Duru, Mehmet Emin
Chaerophyllum species are used as a food additive with their unique aroma and in traditional treatment with beneficial health effects. In this study, various extracts of Chaerophyllum bulbosum L. aerial parts were investigated in terms of the isolation of compounds and ability to inhibit enzyme (AChE, BChE urease, tyrosinase, alpha-amylase, and alpha-glucosidase) activities. The hexane extract showed higher inhibitory activity against all enzymes except urease. Nine compounds, n-octacosane (1), n-heptadecanyl eicosanoate (2), stigmasterol (3), beta-sitosterol-3-O-beta-D-glucopyranoside (4), quercetin-3-O-beta-D-glucopyranoside (5), apigenin-7-O-beta-D-glucopyr-anoside (6), luteolin-7-O-beta-D-glucopyranoside (7), sucrose (8), and luteolin-7-O-alpha-D-glucopyranoside-4 ''-O-beta-D-glucopyranoside (9) were purified from C. bulbosum and their chemical structures were defined by confirming their IR, NMR, and MS data with the literature. Antioxidant and enzyme inhibitory activities of all purified compounds were searched. Compounds 5 and 7 were found to have higher DPPH. and ABTS.+ scavenging activities than standards BHA and alpha-tocopherol. 1 (IC50: 1.63 +/- 0.05 mu g/mL), 2 (IC50: 2.02 +/- 0.13 mu g/mL) and 3 (IC50: 5.36 +/- 0.22 mu g/mL) had higher urease inhibition activity than thiourea (IC50: 7.87 +/- 0.18 mu g/mL) used as a standard. In addition, it was determined that compound 7 (IC50: 352.63 +/- 0.23 mu g/mL) exhibited higher alpha-glucosidase enzyme inhibitory activity as compared to acarbose (IC50: 378.66 +/- 0.14 mu g/mL) while it showed a competitive activity against alpha-amylase. The results reveal that C. bulbosum can be used as a natural source for developing plant-based formulations in fields such as food, pharmaceutical, and cosmetics.
Isolation and Characterization of Chemical Constituents From Chaerophyllum Bulbosum Roots and Their Enzyme Inhibitory and Antioxidant Effects
(Walter De Gruyter Gmbh, 2022) Tel-Çayan, Gülşen; Deveci, Ebru; Molo, Zeynep; Duru, Mehmet Emin; Öztürk, Mehmet
Isolation and bioactive effects of the roots of Chaerophyllum bulbosum L. were firstly investigated herein. Enzyme (acetylcholinesterase, butyrylcholinesterase, urease, alpha-amylase, alpha-glucosidase, and tyrosinase) inhibitory effects of C. bulbosum root extracts were tested. Three known compounds, n-heptadecanyl eicosanoate (1), stigmasterol (2), and beta-sitosterol-3-O-beta-d-glucopyranoside (3) were isolated from C. bulbosum. Antioxidant and enzyme inhibitory effects of isolated compounds were investigated. The hexane extract (IC50: 349.58 +/- 0.06 mu g/mL) displayed a higher alpha-glucosidase inhibitory effect than the standard (IC50: 378.66 +/- 0.14 mu g/mL). The best inhibitory effect was found in compound 2 on AChE (46.40 +/- 0.31%), BChE (56.41 +/- 0.54%), and urease (92.47 +/- 0.11%); compound 1 on alpha-amylase (22.27 +/- 0.61%); and compound 3 on alpha-glucosidase (12.43 +/- 0.25%) and tyrosinase (19.00 +/- 0.16%). All isolated compounds showed moderate antioxidant effects in all assays. This study contributes to the therapeutic uses of Chaerophyllum roots and emphasizes the value of C. bulbosum species for the development of novel therapeutic agents.
Citation - WoS: 3
Citation - Scopus: 7
Isolation and Identification of Compounds From Truffle Reddellomyces Westraliensis and Their Antioxidant, Cytotoxic and Enzyme Inhibitory Activities
(Elsevier Ltd, 2022) Çayan, Fatih; Tel-Çayan, Gülsen; Deveci, Ebru; Duru, Mehmet Emin; Öztürk, Mehmet
Truffles have become one of the world's most important mushrooms owing to their distinctive features. To the best of our knowledge, this is the first study on Reddellomyces westraliensis in terms of isolation and identification of compounds and screening versatile bioactivities. Chromatographic studies on R. westraliensis enabled to isolate brassicasterol (1), ergosta-7,9,22-trien-3?-ol (2), ergosterol peroxide (3), adenosine (4), D-ribitol (5), fumaric acid (6) and mannitol (7). The structures of the compounds were verified by IR, NMR, MS techniques. Antioxidant, cytotoxic and cholinesterase, tyrosinase, urease, ?-amylase and ?-glucosidase inhibitory activities were assessed. The methanol was the suitable extraction solvent high content of phenolic compound (18.94 ± 0.18 ?g PEs/mg extract). Compound 4 showed higher DPPH• (IC50: 8.38 ± 0.40 µg/mL) and ABTS•+ (IC50: 6.74 ± 0.22 µg/mL) scavenging activity than ?-tocopherol and BHA. Compound 3 was observed as a potent inhibitor against AChE (34.03 ± 0.13 %), ?-amylase (34.28 ± 0.36 %) and BChE (66.31 ± 0.08 %). Compounds 1 (IC50: 5.85 ± 0.16 µg/mL) and 3 (IC50: 6.98 ± 0.24 µg/mL) indicated higher urease inhibitory activity than thiourea (IC50: 7.87 ± 0.18 µg/mL). Compounds 4 (9.61 ± 0.92 %) and 7 (21.56 ± 0.74 %) indicated higher ?-glucosidase and tyrosinase inhibitory activity, respectively. Compound 2 exhibited the best cytotoxicity against H1299 (IC50: 38.62 ± 0.46 µg/mL) and MCF-7 (IC50: 24.93 ± 0.80 µg/mL). HPLC-DAD analysis exhibited the existence of fumaric acid (27.20 ± 0.06 µg/g) as the major compound. This study revealed that R. westraliensis can be approved as antioxidant, cytotoxic, and enzyme inhibitor sources in conjunction with its varied bioactive compounds. © 2022 Elsevier Ltd
Citation - WoS: 25
Citation - Scopus: 30
Isolation, Structural Characterization, and Biological Activities of Galactomannans From Rhizopogon Luteolus and Ganoderma Adspersum Mushrooms
(ELSEVIER, 2020) Çayan, Gülsen Tel; Muhammad, Akhtar; Deveci, Ebru; Duru, Mehmet Emin; Öztürk, Mehmet
Polysaccharides are essential compounds that contribute to the biological activities of mushrooms. Two new galactomannans (Galactomannan I and II) were isolated from R. luteolus and G. adspersum. Their structures were characterized using FT-IR, 1D, and 2D-NMR techniques. Both isolated galactomannans I and II mainly include D-mannose and D-galactose in the molar percentages of 0.81:1.0 and 1:1.4, respectively. The GPC calculation demonstrated that the molecular weights are about 5240 and 5090 Da, respectively. Their structures comprise of beta-(1,4)-mannose (Man) backbone units with alpha-(1,6)-galactose (Gal) single unit as a side group. The anticholinesterase activity of galactomannans was tested against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), spectrophotometrically. Antioxidant activity was also measured by six assays (ABTS(center dot+), DPPH center dot, O-2(center dot-), beta-carotene-linoleic acid, metal chelating, and CUPRAC assays). Galactomannan II indicated close inhibitory activity to galantamine against AChE (61.04 +/- 0.45%) and BChE (59.70 +/- 1.15%) at 50 mu g/mL concentration. Nevertheless, both galactomannans showed low antioxidant activity in all tests. This study reveals that mainly, Galactomannan II could be used as a new natural promising anticholinesterase agent. (C) 2020 Elsevier B.V. All rights reserved.
Citation - WoS: 8
Citation - Scopus: 11
Phenolic Profile, Antioxidant and Cholinesterase Inhibitory Activities of Four Trametes Species: T. Bicolor, T. Pubescens, T. Suaveolens, and T. Versicolor
(SPRINGER, 2021) Çayan, Gülsen Tel; Çayan, Fatih; Deveci, Ebru; Duru, Mehmet Emin
Trametes genus is one of the most important medicinal mushroom species in the world. The present study focused on phenolic profile, cholinesterase inhibitory and antioxidant activities of four Trametes species (T. bicolor, T. pubescens, T. suaveolens and T. versicolor). Phenolic profiles of the mushrooms were characterized by HPLC-DAD. ABTS(center dot+) scavenging, beta-carotene-linoleic acid, Cupric-reducing antioxidant capacity (CUPRAC), DPPH center dot scavenging, and metal chelating assays were performed to evaluate antioxidant activities of the extracts. Ellman method was used to test butyrylcholinesterase (BChE) and acetylcholinesterase (AChE) inhibitory activities of the extracts. The most abundant compound was fumaric acid (4.51 +/- 0.10 mu g/g) in T. bicolor, trans-cinnamic acid (0.49 +/- 0.05 mu g/g) in T. pubescens, catechin hydrate in T. suaveolens (0.92 +/- 0.16 mu g/g) and T. versicolor (0.96 +/- 0.19 mu g/g). T. pubescens acetone extract showed the highest antioxidant activity in CUPRAC (A(0.50): 19.26 +/- 0.21 mu g/mL), ABTS(center dot+) (IC50: 3.55 +/- 0.16 mu g/mL), DPPH center dot (85.12 +/- 0.44%)(,) beta-carotene-linoleic acid (IC50: 1.12 +/- 0.41 mu g/mL) assays. The best metal chelating activity was found in T. versicolor hexane extract (56.78 +/- 0.63%). It was determined that T. pubescens hexane extract (IC50: 7.37 +/- 0.55, 15.24 +/- 0.98 mu g/mL, respectively) showed higher AChE and BChE inhibitory activities. The results of this study support the potential use of Trametes species to design new functional drug formulations.