Browsing by Author "Altinok, Bahar Yilmaz"

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Citation - WoS: 12
Citation - Scopus: 16
Characterization of Polysaccharide Extracts of Four Edible Mushrooms and Determination of in Vitro Antioxidant, Enzyme Inhibition and Anticancer Activities
(Amer Chemical Soc, 2024) Deveci, Ebru; Tel-Cayan, Gülsen; Cayan, Fatih; Altinok, Bahar Yilmaz; Aktaş, Sinan
Mushroom polysaccharides are important bioactive compounds derived from mushrooms with various beneficial properties. In this study, the chemical characterization and bioactivities of polysaccharide extracts from four different edible mushrooms, Clavariadelphus truncatus Donk, Craterellus tubaeformis (Fr.) Quel., Hygrophorus pudorinus (Fr.) Fr., and Macrolepiota procera (Scop.) Singer were studied. Glucose (13.24-56.02%), galactose (14.18-64.05%), mannose (2.18-18.13%), fucose (1.21-5.78%), and arabinose (0.04-5.43%) were identified in all polysaccharide extracts by GC-MS (gas chromatography-mass spectrometry). FT-IR (Fourier transform infrared spectroscopy) confirmed the presence of characteristic carbohydrate patterns. H-1 NMR suggested that all polysaccharide extracts had alpha- and beta-d-mannopyranose, d-glucopyranose, d-galactopyranose, alpha-l-arabinofuranose, and alpha-l-fucopyranose residues. Approximate molecular weights of polysaccharide extracts were determined by HPLC (high-performance liquid chromatography). The best antioxidant activity was found in M. procera polysaccharide extract in DPPH center dot (1,1-diphenyl-2-picrylhydrazyl) scavenging (39.03% at 800 mu g/mL), CUPRAC (cupric reducing antioxidant capacity) (A(0.50): 387.50 mu g/mL), and PRAP (phosphomolybdenum reducing antioxidant power) (A(0.50): 384.08 mu g/mL) assays. C. truncatus polysaccharide extract showed the highest antioxidant activity in ABTS(center dot+) scavenging (IC50: 734.09 mu g/mL), beta-carotene-linoleic acid (IC50: 472.16 mu g/mL), and iron chelating (IC50: 180.35 mu g/mL) assays. Significant anticancer activity was found in C. truncatus polysaccharide extract on HT-29 (IC50: 46.49 mu g/mL) and HepG2 (IC50: 48.50 mu g/mL) cell lines and H. pudorinus polysaccharide extract on the HeLa cell line (IC50: 51.64 mu g/mL). Also, H. pudorinus polysaccharide extract possessed prominent AChE (acetylcholinesterase) inhibition activity (49.14% at 200 mu g/mL).
A Chemometric Perspective on Revealing Phytochemical Compounds Associated With Antioxidant and Anti-Cancer Activities of Plants in Türkiye
(Springer, 2025) Deveci, Ebru; Tel-Cayan, Gulsen; Cayan, Fatih; Altinok, Bahar Yilmaz
Medicinal plants serve as raw materials for phytochemicals that constitute the basis of many pharmaceutical drugs. In this regard, this study aimed to investigate phytochemical compounds, antioxidant, and anti-cancer activities of five plants (oat (Avena sativa L.), club moss (Lycopodium clavatum L.), basil (Ocimum basilicium L.), dandelion (Taraxacum officinale L.), and valerian (Valeriana officinalis L.)) from T & uuml;rkiye local plant markets. The phytochemical compounds were identified by HPLC and gallic acid (0.43-2.29 mg/g) was present in all plants. Total phenolic contents (TPC) ranged from 27.53 to 132.89 mu g GAEs/mg extract and total flavonoid contents (TFC) ranged from 14.80 to 42.07 mu g QEs/mg extract. When oat indicated the highest antioxidant activity in DPPH center dot scavenging (IC50: 134.20 mu g/mL), ABTS center dot+ scavenging (IC50: 56.07 mu g/mL) and metal chelating (IC50: 348.04 mu g/mL) assays, basil possessed the highest antioxidant activity in CUPRAC (A0.50: 51.36 mu g/mL) and phosphomolybdenum (A0.50: 45.18 mu g/mL) assays. Also, anti-cancer activity was assessed using the Alamar Blue assay, and oat was recorded as the best anti-cancer active extract on HT-29 (IC50: 53.03 mu g/mL) and HeLa (IC50: 29.17 mu g/mL) cell lines with higher activity than cisplatin (IC50: 31.02 mu g/mL for HeLa). Chemometric analysis was applied for clustering by principal component analysis (PCA) and hierarchical cluster analysis (HCA) which revealed that club moss was distinguished from the other plants club moss was distinguished from other plants. These results propose that the plants studied can be important sources of promising phytochemicals as a starting point for further analysis.