Browsing by Author "Çayan, Fatih"

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Citation - WoS: 13
Citation - Scopus: 15
Chemometric Approaches for the Characterization of the Fatty Acid Composition of Seventeen Mushroom Species
(TAYLOR & FRANCIS INC, 2020) Çayan, Fatih; Deveci, Ebru; Çayan, Gülsen Tel; Duru, Mehmet Emin
Mushrooms have been used since ancient times because of their nutritional, medicinal and economic potential. In this study, fatty acid compositions of seventeen mushroom species, naturally growing in Turkey, were determined by using gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Totally, twenty-four fatty acids were identified in mushroom species. Generally, oleic (6.76-59.25%), linoleic (6.45-61.63%), palmitic (3.57-28.09%) and stearic (0.90-19.77%) acids were identified as the main fatty acids in all studied mushroom species. Principal component analyses (PCA) and hierarchical clustering analyses (HCA) were applied to analyze the chemometry of twenty-four fatty acids of the seventeen mushroom species by using Minitab 16.0 software. The main fatty acid compounds found in the mushrooms and their concentrations have been effective in distinguishing mushroom species from each other. As a result of PCA and HCA analysis, it was determined that Daedalea quercina (DQ) was distinctly separated from other mushroom species in terms of fatty acid composition. This study reveals that the chemometric analysis by PCA and HCA techniques could be used for the classification of the mushroom species according to their fatty acid compositions.
Citation - WoS: 12
Citation - Scopus: 14
Chemometrics Evaluation of Phytochemicals and Antioxidant Activities of the Extracts of Chaerophyllum Bulbosum Roots and Aerial Parts
(TAYLOR & FRANCIS INC, 2022) Çayan, Gülsen Tel; Deveci, Ebru; Çayan, Fatih; Molo, Zeynep; Duru, Mehmet Emin; Yeşil, Yeter
The objective of this study was provide the first report to assess the high-performance liquid chromatography-diode array detection (HPLC-DAD) identification of phytochemicals, the spectrophotometric determination of total phenolics and flavonoids, and antioxidant activities of the root and aerial extracts of Chaerophyllum bulbosum. HPLC-DAD identified the main phytochemical to be rosmarinic acid in the roots in the acetone (CBRA) (13.98 +/- 0.12 mg/g) and the aerial water (CBAW) extract; myricetin (8.30 +/- 0.22 mg/g) in the root methanol extract (CBRM); trans-aconitic acid (6.46 +/- 0.49 mg/g) in the root water extract (CBRW) and fumaric acid in the aerial methanol (CBAM) (17.43 +/- 0.87 mg/g) and water CBAW (18.96 +/- 0.81 mg/g) extracts. The concentrations of total phenolics and flavonoids were 2.00 +/- 0.14-17.79 +/- 0.99 mu g PEs/mg extract and 2.90 +/- 0.00-27.64 +/- 0.37 mu g QEs/mg extract, respectively. The highest antioxidant activity was found in the root acetone extract (CBRA) using the beta-carotene-linoleic (IC50: 172.31 +/- 0.16 mu g/mL) and CUPRAC (A(0.50): 126.00 +/- 0.24 mu g/mL) assays and in the root water extract (CBRW) for DPPH center dot (IC50: 59.51 +/- 0.21 mu g/mL) and metal chelating (IC50: 65.91 +/- 0.36 mu g/mL) assays in the aerial water extract (CBAW) using the ABTS(center dot+) (IC50: 59.51 +/- 0.21 mu g/mL) assay. The relationship between the phytochemicals and antioxidant activities of the extracts was chemometrically determined by principal component analysis (PCA) and hierarchical cluster analysis (HCA).
Citation - WoS: 14
Citation - Scopus: 16
A Comprehensive Study on Phenolic Compounds and Bioactive Properties of Five Mushroom Species Via Chemometric Approach
(WILEY, 2021) Çayan, Fatih; Çayan, Gülsen Tel; Deveci, Ebru; Duru, Mehmet Emin
This study was planned to investigate the phenolic compounds, cholinesterase inhibitory, and antioxidant activities of Chondrostereum purpureum (CP), Hymenochaete rubiginosa (HR), Macrolepiota procera (MP), Phaeolus schweinitzii (PS), and Phellinus igniarius (PI) mushroom species. High-performance liquid chromatography (HPLC-DAD) was used for the characterization of the phenolic compounds. The main compound was identified as fumaric acid in C. purpureum (CP), M. procera (MP), and P. igniarius (PI); protocatechuic acid in H. rubiginosa (HR); 6,7-dihydroxy coumarin in P. schweinitzii (PS). P. schweinitzii methanol (PSM) extract had close or higher activity than standards in ABTS(center dot+) (IC50: 2.96 +/- 0.15 mu g/ml), DPPH center dot (80.56 +/- 0.53%), CUPRAC (A(0.50): 5.26 +/- 0.19 mu g/ml), beta-carotene-linoleic acid (IC50: 1.16 +/- 0.14 mu g/ml) assays. In addition, P. schweinitzii methanol (PSM) extract showed potential inhibitory activity against AChE (56.38 +/- 0.09%). The relationship between the phenolic compounds, antioxidant, and cholinesterase inhibitory activity results and the mushroom species were examined using principal component analysis (PCA) and hierarchical cluster analysis (HCA). Practical applications This study revealed that studied mushroom species have significant antioxidant and cholinesterase inhibitory activities and are found to be rich in phenolic compounds. The mushroom species were clustered with their antioxidant and cholinesterase inhibitory activities and phenolic compounds data according to the PCA and HCA analysis. Overall, the results described in this study increase the contribution and emphasis on the potential use of these mushrooms as a source of versatile biological agents.
Citation - WoS: 10
Citation - Scopus: 16
A Detailed Study on Multifaceted Bioactivities of the Extracts and Isolated Compounds From Truffle Reddellomyces Parvulosporus
(WILEY, 2022) Çayan, Fatih; Çayan, Gülsen Tel; Deveci, Ebru; Duru, Mehmet Emin; Türk, Mustafa
Mushrooms and truffles are attracting attention as a new generation of biotherapeutics. In the current study, isolation, phenolic and organic acid composition, and antioxidant, cytotoxic, anticholinesterase activities of truffle Reddellomyces parvulosporus were examined. Four known compounds (brassicasterol (1), ergosterol peroxide (2), fumaric acid (3) and mannitol (4)) were isolated with the combination of chromatographic techniques. Fumaric acid (54.74 +/- 0.85 mu g g(-1)) was found as the major compound by HPLC-DAD. All isolated compounds were bioassayed for antioxidant, cytotoxic, anticholinesterase, anti-tyrosinase, anti-urease, anti-alpha-glucosidase and anti-alpha-amylase activities. Compound 1 indicated notable cytotoxic activity on MCF-7 (IC50: 38.08 +/- 0.75 mu g mL(-1)) and compound 3 on H1299 (IC50: 62.37 +/- 0.75 mu g mL(-1)). Also, compounds 1 (84.55 +/- 1.14%) and 2 (84.90 +/- 0.10%) showed higher anti-urease activity than thiourea (78.57 +/- 0.22%), while compound 2 (66.31 +/- 0.08%) displayed near-standard anti-BChE activity. Also, being the first to emphasise the potential of R. parvulosporus as a natural food additive, this study evidenced its medicinal importance by revealing bioactive compounds and properties.
Citation - WoS: 21
Citation - Scopus: 22
Hplc-Dad Characterization of Phenolic Profile and in Vitro Antioxidant, Anticholinesterase, and Antidiabetic Activities of Five Mushroom Species From Turkey
(Springer Heidelberg, 2021) Çayan, Fatih; Tel-Çayan, Gülşen; Deveci, Ebru; Duru, Mehmet Emin
In this study, Daedalea quercina (L.) Pers., Hydnum repandum L., Inonotus radiatus (Sowerby) P. Karst., Omphalotus olearius (DC.) Singer, and Schizophyllum commune Fr. hexane and methanol extracts were subjected to the spectrophotometric assays for antioxidant and enzyme inhibitory activities, which are linked with human diseases that are very prevalent in recent years. Additionally, phenolic compounds of the mushrooms were quantified by HPLC-DAD. The best antioxidant activity was found in H. repandum methanol extract (IC50: 12.04 +/- 0.24 mu g/mL) in the beta -carotene-linoleic assay; I. radiatus methanol extract in DPPH center dot (81.22 +/- 0.50%), ABTS(center dot+) (IC50: 73.47 +/- 0.18 mu g/mL), and CUPRAC (A(0.50): 88.21 +/- 0.02 mu g/mL) assays; S. commune hexane extract (53.36 +/- 0.89%) in the metal chelating assay. O. olearius hexane extract was found as the best inhibitor against AChE (71.58 +/- 0.28%) and BChE (67.30 +/- 0.15%). When I. radiatus methanol (95.88 +/- 0.74%) and H. repandum hexane (95.75 +/- 0.16%) extracts showed close alpha -amylase inhibitory activity to acarbose (96.68 +/- 0.08%), D. quercina methanol extract (70.79 +/- 0.34%) had higher alpha -glucosidase inhibitory activity than acarbose (67.01 +/- 2.28%). Among 16 phenolic compounds analyzed, gallic acid (0.02 +/- 0.01-0.23 +/- 0.01 mu g/g) was detected in all studied mushrooms. This study provides that investigated mushrooms can be used for further research, which can lead to the development of new natural remedies to alleviate complications related to oxidative stress, diabetes, and neurological diseases.
Citation - WoS: 83
Citation - Scopus: 98
Identification and Quantification of Phenolic Acid Compounds of Twenty-Six Mushrooms by Hplc-Dad
(SPRINGER, 2020) Çayan, Fatih; Deveci, Ebru; Çayan, Gülsen Tel; Duru, Mehmet Emin
Phenolic acids are found in different foods in the human diet, for example mushrooms. Determination of phenolic acids is important because of their relationship to their role in disease prevention due to their bioactive properties. In this study, the phenolic acid profile of 26 mushroom species was analyzed by using high-performance liquid chromatography method coupled with photodiode array detector (HPLC-DAD) and 16 phenolic acid compounds were identified. The chromatographic separation was performed using Intertsil ODS-3 reverse phase C-18 column (5 mu m, 250 mm x 4.6 mm i.d), gradient solvent system with 1.5 mL/min flow rate and detected at 280 nm. The coefficient of determination (R-2) was in the range of 0.9965-0.9999. Limit of detection and quantification ranged from 0.001-0.970 to 0.001-2.940 mu g/L, respectively. The phenolic compounds were characterized according to their retention times and UV data were compared with commercial standards. S. granulatus (71.79 mu g/g) and L. nuda (68.38 mu g/g) revealed the highest concentration of total phenolic compounds among the studied mushrooms. Gallic acid was found as the major phenolic compound in R. aurora (2.96 +/- 0.56 mu g/g) while 6,7-dihydroxy coumarin was identified as major phenolic compounds in A. tabescens (2.07 +/- 0.25 mu g/g) and L. leucothites (9.02 +/- 0.87 mu g/g). Fumaric acid was found as the most abundant compounds in 16 out of 26 mushrooms. Catechin hydrate was identified as major phenolic compounds in the rest of mushrooms. This method provided a beneficial standardization procedure of phenolic acid compounds in mushroom samples.
Citation - WoS: 46
Citation - Scopus: 56
Inhibitory Activities of Medicinal Mushrooms on Alpha-Amylase and Alpha-Glucosidase Related To Type 2 Diabetes
(ELSEVIER, 2021) Deveci, Ebru; Çayan, Fatih; Çayan, Gülsen Tel; Duru, Mehmet Emin
Mushrooms have been used as a primary source of medicines since ancient times due to the presence of bioactive compounds. Enzyme inhibition is an important field of pharmaceutical research that provides insight into the discovery of a large variety of drugs that are useful in treating many diseases. The inhibitory activities of the hexane and methanol extracts of twenty-four medicinal mushrooms on alpha-amylase and alpha-glucosidase enzymes related to type 2 diabetes were evaluated in this study. Among all studied mushroom extracts, C. rutilus hexane extract (IC50: 0.05 +/- 0.01 mg/mL) demonstrated the highest inhibitory activity on alpha-amylase while P. ostreatus hexane (IC50: 0.10 +/- 0.01 mg/mL) showed the highest inhibitory activity on alpha-glucosidase. The hexane extracts of C. rutilus (98.81 +/- 0.01 %), G. adspersum (96.96 +/- 0.47 %), G. sepiarium (96.89 +/- 1.01 %), and S. granulatus (97.74 +/- 0.27 %) displayed higher inhibitory activity on alpha-amylase enzyme than acarbose at 1.00 mg/mL concentration. Also, the hexane extracts of P. ostreatus (IC50: 0.10 +/- 0.01 mg/mL), M. procera (IC50: 0.11 +/- 0.01 mg/mL), P. schweinitzii (IC50: 0.14 +/- 0.01 mg/mL), L. gentianeus (IC50: 0.22 +/- 0.02 mg/mL), P. pini (IC50: 0.22 +/- 0.03 mg/mL) and the methanol extracts of T. pubescens (IC50: 0.12 +/- 0.02 mg/mL) and G. adspersum (IC50: 0.20 +/- 0.04 mg/mL) showed higher inhibitory activity on alpha-glucosidase enzyme than acarbose (IC50: 0.37 +/- 0.01 mg/mL). This study with the reported results supported the use of mushrooms in the pharmaceutical industries as natural antidiabetic agents through key enzyme inhibition. (C) 2020 SAAB. Published by Elsevier B.V. All rights reserved.
Citation - WoS: 3
Citation - Scopus: 7
Isolation and Identification of Compounds From Truffle Reddellomyces Westraliensis and Their Antioxidant, Cytotoxic and Enzyme Inhibitory Activities
(Elsevier Ltd, 2022) Çayan, Fatih; Tel-Çayan, Gülsen; Deveci, Ebru; Duru, Mehmet Emin; Öztürk, Mehmet
Truffles have become one of the world's most important mushrooms owing to their distinctive features. To the best of our knowledge, this is the first study on Reddellomyces westraliensis in terms of isolation and identification of compounds and screening versatile bioactivities. Chromatographic studies on R. westraliensis enabled to isolate brassicasterol (1), ergosta-7,9,22-trien-3?-ol (2), ergosterol peroxide (3), adenosine (4), D-ribitol (5), fumaric acid (6) and mannitol (7). The structures of the compounds were verified by IR, NMR, MS techniques. Antioxidant, cytotoxic and cholinesterase, tyrosinase, urease, ?-amylase and ?-glucosidase inhibitory activities were assessed. The methanol was the suitable extraction solvent high content of phenolic compound (18.94 ± 0.18 ?g PEs/mg extract). Compound 4 showed higher DPPH• (IC50: 8.38 ± 0.40 µg/mL) and ABTS•+ (IC50: 6.74 ± 0.22 µg/mL) scavenging activity than ?-tocopherol and BHA. Compound 3 was observed as a potent inhibitor against AChE (34.03 ± 0.13 %), ?-amylase (34.28 ± 0.36 %) and BChE (66.31 ± 0.08 %). Compounds 1 (IC50: 5.85 ± 0.16 µg/mL) and 3 (IC50: 6.98 ± 0.24 µg/mL) indicated higher urease inhibitory activity than thiourea (IC50: 7.87 ± 0.18 µg/mL). Compounds 4 (9.61 ± 0.92 %) and 7 (21.56 ± 0.74 %) indicated higher ?-glucosidase and tyrosinase inhibitory activity, respectively. Compound 2 exhibited the best cytotoxicity against H1299 (IC50: 38.62 ± 0.46 µg/mL) and MCF-7 (IC50: 24.93 ± 0.80 µg/mL). HPLC-DAD analysis exhibited the existence of fumaric acid (27.20 ± 0.06 µg/g) as the major compound. This study revealed that R. westraliensis can be approved as antioxidant, cytotoxic, and enzyme inhibitor sources in conjunction with its varied bioactive compounds. © 2022 Elsevier Ltd
Citation - WoS: 8
Citation - Scopus: 11
Phenolic Profile, Antioxidant and Cholinesterase Inhibitory Activities of Four Trametes Species: T. Bicolor, T. Pubescens, T. Suaveolens, and T. Versicolor
(SPRINGER, 2021) Çayan, Gülsen Tel; Çayan, Fatih; Deveci, Ebru; Duru, Mehmet Emin
Trametes genus is one of the most important medicinal mushroom species in the world. The present study focused on phenolic profile, cholinesterase inhibitory and antioxidant activities of four Trametes species (T. bicolor, T. pubescens, T. suaveolens and T. versicolor). Phenolic profiles of the mushrooms were characterized by HPLC-DAD. ABTS(center dot+) scavenging, beta-carotene-linoleic acid, Cupric-reducing antioxidant capacity (CUPRAC), DPPH center dot scavenging, and metal chelating assays were performed to evaluate antioxidant activities of the extracts. Ellman method was used to test butyrylcholinesterase (BChE) and acetylcholinesterase (AChE) inhibitory activities of the extracts. The most abundant compound was fumaric acid (4.51 +/- 0.10 mu g/g) in T. bicolor, trans-cinnamic acid (0.49 +/- 0.05 mu g/g) in T. pubescens, catechin hydrate in T. suaveolens (0.92 +/- 0.16 mu g/g) and T. versicolor (0.96 +/- 0.19 mu g/g). T. pubescens acetone extract showed the highest antioxidant activity in CUPRAC (A(0.50): 19.26 +/- 0.21 mu g/mL), ABTS(center dot+) (IC50: 3.55 +/- 0.16 mu g/mL), DPPH center dot (85.12 +/- 0.44%)(,) beta-carotene-linoleic acid (IC50: 1.12 +/- 0.41 mu g/mL) assays. The best metal chelating activity was found in T. versicolor hexane extract (56.78 +/- 0.63%). It was determined that T. pubescens hexane extract (IC50: 7.37 +/- 0.55, 15.24 +/- 0.98 mu g/mL, respectively) showed higher AChE and BChE inhibitory activities. The results of this study support the potential use of Trametes species to design new functional drug formulations.