Mustafa, AbdurrahmanAlSarori, AhmedAkyildiz, HasanKaya, Ismail CihanKaya, Gulcihan Guzel2025-08-102025-08-1020252366-3987https://doi.org/10.1002/adtp.202500119https://hdl.handle.net/20.500.13091/10582FeS2, a member of metal chalcogenide semiconductors, is a cheap and available material with distinguishable photothermal activity under light irradiation. However, its photodynamic properties have to be improved for practical phototherapy applications. Therefore, in this study, FeS2/WS2 p-n junctions, comprising varying amounts of WS2, are synthesized using the simple hot injection method. Establishment of the heterostructure is verified using X-ray diffraction (XRD), scanning transmission electron microscopy (STEM), energy-dispersive X-ray spectroscopy (EDX), and X-ray photoelectron spectroscopy (XPS) analysis. p-n junction formation is further validated via calculations based on ultraviolet-visible (UV-vis.) spectrophotometer and XPS data. Photothermal and photodynamic properties of the samples are examined considering various aspects. The FeS2/WS2 heterostructure provides a heating response above 50 degrees C with a high photothermal conversion efficiency of 52.6%. The reactive oxygen species (ROS) formation ability is observed to depend on the material concentration and O2 center dot & oline; is determined as the primary reactive oxygen species. The in vitro antibacterial activity of the samples is tested against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) bacteria as a function of material concentration. At all concentrations, the FeS2/WS2 heterostructure exhibits higher activity than that of FeS2 nanoparticles. The efficiency of the sample against S. aureus and E. coli is calculated to be 100% and 99.4% respectively, at a low material concentration of 100 mu g mL-1.eninfo:eu-repo/semantics/closedAccessAntibacterial ActivityFeS2HeterojunctionPhototherapyWS2Article10.1002/adtp.2025001192-s2.0-105011866049