Bayram, Sarıipek, F.2023-12-262023-12-2620240141-8130https://doi.org/10.1016/j.ijbiomac.2023.128330https://hdl.handle.net/20.500.13091/4945The increasing prevalence of multi-drug resistant bacteria poses a significant threat to public health, especially in wound infections. Developing new bactericidal agents and treatment strategies is crucial to address this issue. In this study, biopolymeric nanofibrous scaffolds containing green-synthesized silver nanoparticles (AgNPs) with curcumin (CUR) were evaluated as antimicrobial materials for wound healing therapy. Firstly, CUR was utilized to synthesize AgNPs, which were then analyzed using various analytical methods. The microstructural analysis revealed that the biogenic AgNPs, which had a spherical shape and an average size of 19.83 nm, were uniformly anchored on PHB/CTS nanofibers. Then, the AgNPs with various content (0.25–1%wt) were incorporated into PHB/CTS matrix to enhance its wettability, thermal and bactericidal behaviors. The nanofibrous scaffolds were characterized by FT-IR, FE-SEM, TGA analysis and water contact angle measurement. Overall, the addition of CUR-AgNPs to the PHB/CTS matrix led to a reduction in fiber diameter, enhanced hydrophilicity and improved thermal properties. Additionally, antibacterial activity against Staphylococcus aureus and Escherichia coli was performed on samples of AgNPS and PHB/CTS/CUR-Ag. The synthesized AgNPs showed antibacterial activity against both microorganisms, especially against S. aureus. Higher concentrations of AgNPs in nanofibers led to a significant reduction in bacterial colony formation. The results displayed that PHB/CTS/CUR-AgNPs nanofibrous scaffolds could be a promising material for the biomedical applications such as wound healing. © 2023 Elsevier B.V.eninfo:eu-repo/semantics/closedAccessAntibacterial activityBiogenic silver nanoparticleBiopolymeric nanofibrous scaffoldsBiopolymersContact angleDrug deliveryEscherichia coliHealth risksMedical applicationsMetal nanoparticlesNanofibersScaffoldsScaffolds (biology)Synthesis (chemical)% reductionsAnti-bacterial activityBiogenic silver nanoparticleBiogenicsBiopolymeric nanofibrous scaffoldCurcuminmatrixNanofibrous scaffoldsSynthesisedWound healingSilver nanoparticlesbiogenic silver nanoparticlebiopolymerchitosanchitosan nanoparticlecurcuminnanofiberpoly(3 hydroxybutyric acid)silver nanoparticleunclassified drugantibacterial activityArticlebacterial colonizationbiopolymeric nanofibrous scaffoldcontact anglecontrolled studydrug delivery systemEscherichia colifield emission scanning electron microscopyFourier transform mass spectrometrygreen chemistryhydrophilicitynanofabricationnonhumanparticle sizeStaphylococcus aureusstructure analysissynthesisthermogravimetrytransmission electron microscopyultraviolet visible spectrophotometrywettabilitywound healingX ray diffractionArticle10.1016/j.ijbiomac.2023.1283302-s2.0-85178362906