Please use this identifier to cite or link to this item:
https://hdl.handle.net/20.500.13091/3201
Title: | Electrochemical immunosensor for individual and simultaneous determination of Cytokeratin fragment antigen 21-1 and Neuron-specific enolase using carbon dots-decorated multiwalled carbon nanotube electrode | Authors: | Filik, Hayati Avan, Asiye Aslihan Puntar, Nilay Altas Özyürek, Mustafa Çakıcı, Maside Guengor, Zeynep Banu Kamış, Handan |
Keywords: | Cytokeratin fragment antigen 21-1 Neuron-specific enolase Immunosensor Voltammetry Serum analysis Multiple Tumor-Markers Cell Lung-Cancer 4 Biomarkers 3d Graphene Immunoassay Biosensors Nanocomposites Nanoparticles Oxidation Platform |
Publisher: | Elsevier | Abstract: | In this approach, we fabricated a sandwich-type electrochemical immunosensor for individual and simultaneous sensing of cytokeratin fragment antigen 21-1 (CYFRA 21-1) and neuron-specific enolase (NSE) using the multiple-label concept. In this report, carbon dots (CDs) were prepared from citric acid and ethylenediamine (EDA). A hybrid EDA-CDs-MWCNTs composites were prepared and then decorated with gold nanoparticles (AuNPs). The obtained hybrid composites were used as an immunosensing platform. Furthermore, AuNPs decorated EDA-CDs-MWCNTs was carefully loaded with reactive dye (orange G and carminic acid) and utilized as signal labels. The surface morphology and the electrochemical properties of the modified SPCE were studied carefully. The linear range for the individual and simultaneous detection was 0.002-10 ng mL-1 for the CYFRA 21-1 and NSE and the detection limits (LODs) were 0.22 pg mL-1 and 0.15 pg mL-1 for the individual detection while 0.25 pg mL-1 and 0.20 pg mL-1 for simultaneous detection, respectively. This developed immunoassay was utilized for the assessment of two markers in the serum sample with reasonable consequences. | URI: | https://doi.org/10.1016/j.microc.2022.107990 https://doi.org/10.1016/j.microc.2022.107990 https://hdl.handle.net/20.500.13091/3201 |
ISSN: | 0026-265X 1095-9149 |
Appears in Collections: | Mühendislik ve Doğa Bilimleri Fakültesi Koleksiyonu Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collections WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collections |
Files in This Item:
File | Size | Format | |
---|---|---|---|
1-s2.0-S0026265X22008189-main.pdf Until 2030-01-01 | 4.69 MB | Adobe PDF | View/Open Request a copy |
CORE Recommender
WEB OF SCIENCETM
Citations
6
checked on Aug 31, 2024
Page view(s)
102
checked on Aug 26, 2024
Download(s)
8
checked on Aug 26, 2024
Google ScholarTM
Check
Altmetric
Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.